primary rat anti mouse primary monoclonal antibodies against cd68  (Bio-Rad)

Journal: Biomedicines

Article Title: Increased Myocardial MAO-A, Atrogin-1, and IL-1β Expression in Transgenic Mice with Pancreatic Carcinoma—Benefit of MAO-A Inhibition for Cardiac Cachexia

doi: 10.3390/biomedicines12092009

Figure Lengend Snippet: QuantiTect primer assays used for qPCR.

Article Snippet: Transversal or longitudinal cryo-sections of the ventricular left myocardium were fixed by 5% paraformaldehyde (PFA) for 10 min, blocked with 1% normal swine serum (NSS) at 37 °C, and incubated with primary rabbit anti-mouse polyclonal antibodies against atrogin-1 (FBXO32, MAFbx, 1:200, ab74023, Abcam, Cambridge, UK), against IL-1β (1:50, ab9722, Abcam, Cambridge, UK), TNF (1:100, ab6671, Abcam, Cambridge, UK) or against COX2 (1:200, ab15191, Abcam, Cambridge, UK), and primary rat anti-mouse primary monoclonal antibodies against CD68 (1:50, MCA1957, AbD Serotec, Kidlington, UK).

Techniques: Amplification

Journal: Biomedicines

Article Title: Increased Myocardial MAO-A, Atrogin-1, and IL-1β Expression in Transgenic Mice with Pancreatic Carcinoma—Benefit of MAO-A Inhibition for Cardiac Cachexia

doi: 10.3390/biomedicines12092009

Figure Lengend Snippet: Expression of pro-atrophic, -inflammatory, -angiogenic, and -apoptotic transcripts in left myocardium of WT-HH, CA, and CA-HH mice relative to the group of untreated WT mice.

Article Snippet: Transversal or longitudinal cryo-sections of the ventricular left myocardium were fixed by 5% paraformaldehyde (PFA) for 10 min, blocked with 1% normal swine serum (NSS) at 37 °C, and incubated with primary rabbit anti-mouse polyclonal antibodies against atrogin-1 (FBXO32, MAFbx, 1:200, ab74023, Abcam, Cambridge, UK), against IL-1β (1:50, ab9722, Abcam, Cambridge, UK), TNF (1:100, ab6671, Abcam, Cambridge, UK) or against COX2 (1:200, ab15191, Abcam, Cambridge, UK), and primary rat anti-mouse primary monoclonal antibodies against CD68 (1:50, MCA1957, AbD Serotec, Kidlington, UK).

Techniques: Expressing

Journal: Biomedicines

Article Title: Increased Myocardial MAO-A, Atrogin-1, and IL-1β Expression in Transgenic Mice with Pancreatic Carcinoma—Benefit of MAO-A Inhibition for Cardiac Cachexia

doi: 10.3390/biomedicines12092009

Figure Lengend Snippet: CD68+ cells in the left ventricular myocardium of WT, WT-HH, CA, and CA-HH mice. ( a ) Percentage CD68+ cells. Data are given as mean ± SEM. Two-factorial ANOVA detected no significant impact of factor CA or factor HH treatment; however, revealed a significant interaction ( p ≤ 0.05) between these factors within the total study population. * p < 0.05 CA vs. WT. # p < 0.05 WT-HH vs. WT. ( b ) Representative photos of left myocardial cross-sections of WT, WT-HH, CA, and CA-HH mice showing CD68+ cells (arrows). ( c ) X-fold expression of CD68 transcripts in WT-HH, CA, and CA-HH relative to WT mice as assessed in pooled samples.

Article Snippet: Transversal or longitudinal cryo-sections of the ventricular left myocardium were fixed by 5% paraformaldehyde (PFA) for 10 min, blocked with 1% normal swine serum (NSS) at 37 °C, and incubated with primary rabbit anti-mouse polyclonal antibodies against atrogin-1 (FBXO32, MAFbx, 1:200, ab74023, Abcam, Cambridge, UK), against IL-1β (1:50, ab9722, Abcam, Cambridge, UK), TNF (1:100, ab6671, Abcam, Cambridge, UK) or against COX2 (1:200, ab15191, Abcam, Cambridge, UK), and primary rat anti-mouse primary monoclonal antibodies against CD68 (1:50, MCA1957, AbD Serotec, Kidlington, UK).

Techniques: Expressing

Journal: Neuron

Article Title: Reorganization of adolescent prefrontal cortex circuitry is required for mouse cognitive maturation

doi: 10.1016/j.neuron.2023.10.024

Figure Lengend Snippet: Dendritic arborization complexity and spine density of prefrontal layer 2/3 pyramidal neurons throughout late development (A) Characteristic photographs of tDimer2-expressing L2/3 PYRs within the PL displayed together with average heatmaps of all overlayed dendrites for each age group. (B) Dendritic intersections within a 200 μm radius from the soma center of prelimbic L2/3 PYRs (n = 12 mice, 71 neurons). Color-striped bars indicate significant difference. (C) Violin plots displaying the total branch length (left) and number of branches (right) of the cells analyzed in (B). (D) Violin plots displaying spine densities of basal (i.e., originating from the soma; left, n = 17 mice, 60 neurons, 358 dendrites) and distal (i.e., originating from the apex/apical dendrite; right, n = 17 mice, 60 neurons, 251 dendrites) dendrites of L2/3 PYRs within the PL. (E) Characteristic photographs of basal dendrites from each age group. For line plots in (B), data are presented as mean ± SEM. Data of violin plots are presented as median with 25 th and 75 th percentile. ∗ p < 0.05, ∗∗ p < 0.01, ∗∗∗ p < 0.001. See also Figure S6 and Table S1 for detailed statistics. (F) Characteristic photographs of Iba1 immunostainings in the PL of each age group. (G) Violin plot displaying the densities of Iba1-positive cells within the PL (n = 25 mice, 800 stacks). (H) Violin plots displaying roundness, cell spread, cell area, and cell perimeter for Iba1-positive cells within the PL (n = 25 mice, 26,707 cells). (I) Characteristic reconstructions of processed raw images of Iba1-positive cells (green) containing CD68- (blue) and PSD95- (red) positive puncta of L2/3 PYRs from each age group. Pink corresponds to an overlay of all three colors. (J) Bar diagrams displaying the percentage of microglia with colocalized puncta (n = 17 mice, 625 cells). (K) Violin plots displaying volume of vesicles (left) and inclusions (right), quantified on colocalized Iba1-, CD68- or Iba1-, CD68-, and PSD95-positive pixel, respectively (n = 17 mice, 625 cells). (L) Same as (K) for the number of inclusions. (M) Violin plot displaying the phagocytic ratio (i.e., MI of colocalized PSD95 vs. solely CD68 puncta, n = 17 mice, 625 cells). For line plots in (B), data are presented as mean ± SEM. For (K) and (L), data were normalized to Iba1 volume. Data of violin plots are presented as median with 25 th and 75 th percentile. ∗ p < 0.05, ∗∗ p < 0.01, ∗∗∗ p < 0.001. See also Table S1 for detailed statistics.

Article Snippet: Rat monoclonal primary antibody against mouse CD68 , Bio-Rad , Cat# MCA1957GA, RRID: AB_324217.

Techniques: Expressing

Journal: Neuron

Article Title: Reorganization of adolescent prefrontal cortex circuitry is required for mouse cognitive maturation

doi: 10.1016/j.neuron.2023.10.024

Figure Lengend Snippet: Adult cognitive abilities and prefrontal morphology after microglia manipulation during adolescence (A) Left, color-coded heatmaps of the time spent at different locations within the open-field arena by Control I (left) and PLX I (right) P56–P60 mice. Right, violin plots displaying relative time spent in the center (given as %, left) and relative resting time (given as %, right) in the open-field arena (n = 41 mice). (B) Violin plots displaying grooming duration (left) and frequency (right) in the open-field arena (n = 41 mice). (C) Left, color-coded heatmaps of the time spent at different locations within the eight-arm maze by Control I (left) and PLX I (right) P56–P60 mice during all trials. Right, line plot displaying the number of working memory (left) and reference (right) errors for all 12 trials. Violin plots displaying the normalized slope of the fitted working memory (left) and reference memory (right) performance of individual mice (n = 14 mice). Color-striped bars indicate significant difference. (D) Left, schematic of the experimental protocol during odor discrimination. Right, violin plots displaying the number of trials (left) and average trial latency (right) required by each mouse to reach criterium (n = 27 mice). (E) Left, schematic of the experimental protocol during odor reversal. Right, violin plots displaying the number of trials (left), average trial latency (middle), and total errors (given as %, right) required by each mouse to reach criterium (n = 27 mice). (F) Characteristic photographs of tDimer2-expressing L2/3 PYRs within the PL of Control I (left) and PLX I (right) P56–P60 mice. (G) Left, dendritic intersections within a 200 μm radius from the soma center of prelimbic L2/3 PYRs (n = 6 mice, 37 neurons). Color-striped bars indicate significant difference. Right, violin plots displaying the total branch length (left) and the number of branches (right) of the cells analyzed left. (H) Violin plots displaying spine densities of basal (i.e., originating from the soma; left, n = 6 mice, 24 neurons, dendrites) and distal (i.e., originating from the apex/apical dendrite; right, n = 6 mice, 24 neurons, dendrites) dendrites of L2/3 PYRs within the PL. (I) Violin plots displaying the densities (left, n = 6 mice, 200 stacks) and roundness (right, n = 6 mice, 5504 cells) of Iba1-positive cells within the PL. (J) Characteristic reconstructions of processed raw images of Iba1-positive cells (green) containing CD68- (blue) and PSD95- (red) positive puncta of L2/3 PYRs from Control I (left) and PLX I (right) P58–P60 mice. Pink corresponds to an overlay of all three colors. (K) Left, violin plots displaying volume of inclusions (left) and the number of inclusions (right), quantified on colocalized Iba1-, CD68-, and PSD95-positive pixel (n = 6 mice, 270 cells). Right, bar diagrams displaying the percentage of microglia with colocalized puncta. For line plots in (C) and (G), data are presented as mean ± SEM. Data of violin plots are presented as median with 25 th and 75 th percentiles. ∗ p < 0.05, ∗∗ p < 0.01, ∗∗∗ p < 0.001. See also Figures S7 and as well as Table S1 for detailed statistics.

Article Snippet: Rat monoclonal primary antibody against mouse CD68 , Bio-Rad , Cat# MCA1957GA, RRID: AB_324217.

Techniques: Control, Expressing

Journal: Neuron

Article Title: Reorganization of adolescent prefrontal cortex circuitry is required for mouse cognitive maturation

doi: 10.1016/j.neuron.2023.10.024

Figure Lengend Snippet: Long-lasting functional, behavioral, and morphological consequences of microglia manipulation during adolescence (A) Schematic of the experimental procedure. (B) Left, power spectra of LFP activity recorded in the PL of P98–P102 Control II and PLX II mice. Right, scatterplot of peak frequencies as a function of peak amplitudes for 1–12 and 12–100 Hz bands, respectively (n = 11 mice, 22 recordings). (C) Same as (B) for LFP activity recorded in the S1 (n = 11 mice, 22 recordings). (D) Top, color-coded heatmaps of the time spent at different locations within the open-field arena by Control II (left) and PLX II (right) P98–P102 mice. Bottom, violin plots displaying the relative time spent in the center (given as %, left), relative resting time (given as % middle), and grooming frequency (right) in the open-field arena (n = 24 mice). (E) Left, schematic of the experimental protocol of spontaneous alternations monitoring. Right, violin plots displaying the number of entries (left) and percentage of alternations (right) in the Y-maze (n = 24 mice). (F) Left, schematic of the experimental protocol during odor discrimination. Right, violin plots displaying the number of trials (left) and average trial latency (right) required by each mouse to reach criterium (n = 24 mice). (G) Left, schematic of the experimental protocol during odor reversal. Right, violin plots displaying the number of trials (left), average trial latency (middle), and total errors (given as %, right) required by each mouse to reach criterium (n = 24 mice). (H) Characteristic photographs of tDimer2-expressing L2/3 PYRs within the PL of Control II (left) and PLX II (right) P98–P102 mice. (I) Left, dendritic intersections within a 200 μm radius from the soma center of prelimbic L2/3 PYRs (n = 6 mice, 36 neurons). Color-striped bars indicate significant difference. Right, violin plots displaying the total branch length (left) and number of branches (right) of the cells analyzed left. (J) Violin plots displaying spine densities of basal (i.e., originating from the soma; left, n = 6 mice, 24 neurons, 121 dendrites) and distal (i.e., originating from the apex/apical dendrite; right, n = 6 mice, 24 neurons, 101 dendrites) dendrites of L2/3 PYRs within the PL. (K) Violin plots displaying the densities (left, n = 6 mice, 200 stacks) and roundness (right, n = 6 mice, 6,149 cells) of Iba1-positive cells within the PL. (L) Characteristic reconstructions of processed raw images of Iba1-positive cells (green) containing CD68- (blue) and PSD95- (red) positive puncta of L2/3 PYRs from Control II (left) and PLX II (right) P98–P102 mice. Pink corresponds to an overlay of all three colors. (M) Left, violin plots displaying volume of inclusions (left) and the number of inclusions (right), quantified on colocalized Iba1-, CD68-, and PSD95-positive pixel (n = 6 mice, 252 cells). Right, bar diagrams displaying the percentage of microglia with colocalized puncta. For line plots in (B), (C), and (I), data are presented as mean ± SEM. Data of violin plots are presented as median with 25 th and 75 th percentiles. ∗ p < 0.05, ∗∗ p < 0.01, ∗∗∗ p < 0.001. See also Table S1 for detailed statistics.

Article Snippet: Rat monoclonal primary antibody against mouse CD68 , Bio-Rad , Cat# MCA1957GA, RRID: AB_324217.

Techniques: Functional Assay, Activity Assay, Control, Expressing

Journal: Neuron

Article Title: Reorganization of adolescent prefrontal cortex circuitry is required for mouse cognitive maturation

doi: 10.1016/j.neuron.2023.10.024

Figure Lengend Snippet:

Article Snippet: Rat monoclonal primary antibody against mouse CD68 , Bio-Rad , Cat# MCA1957GA, RRID: AB_324217.

Techniques: Virus, Recombinant, Software, Imaging, Electroporation, Microscopy